Size‐Controlled Formation of a Calcium Phosphate‐Based Organic–Inorganic Hybrid Vector for Gene Delivery Using Poly(ethylene glycol)‐block‐poly(aspartic acid)
- 19 April 2004
- journal article
- research article
- Published by Wiley in Advanced Materials
- Vol. 16 (8) , 699-702
- https://doi.org/10.1002/adma.200305782
Abstract
Poly(ethylene glycol) (PEG)‐covered hybrid particles (see Figure) of calcium phosphate (CaP), and plasmid DNA (pDNA) have been prepared in a size‐controllable manner in the presence of a block copolymer. The particles show a substantial colloidal stability as well as tolerance of entrapped pDNA against nuclease attack in the medium, and achieve a significantly higher gene expression compared to conventional precipitates.Keywords
This publication has 16 references indexed in Scilit:
- Parallel Synthesis and Biophysical Characterization of a Degradable Polymer Library for Gene DeliveryJournal of the American Chemical Society, 2003
- Block Copolymer Self-Assembly into Monodispersive Nanoparticles with Hybrid Core of Antisense DNA and Calcium PhosphateLangmuir, 2002
- Polyion Complex Micelles with Reactive Aldehyde Groups on Their Surface from Plasmid DNA and End-Functionalized Charged Block CopolymersMacromolecules, 1999
- Water-Soluble Polyion Complex Associates of DNA and Poly(ethylene glycol)−Poly(l-lysine) Block CopolymerBioconjugate Chemistry, 1997
- Transfecting Mammalian Cells: Optimization of Critical Parameters Affecting Calcium-Phosphate Precipitate FormationNucleic Acids Research, 1996
- A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.Proceedings of the National Academy of Sciences, 1995
- Administration of Oligonucleotides to Cultured Cells by Calcium Phosphate Precipitation MethodAnalytical Biochemistry, 1993
- Organoapatites: Materials for artificial bone. I. Synthesis and microstructureJournal of Biomedical Materials Research, 1992
- The adsorption of acidic amino acids and homopolypeptides on hydroxyapatiteJournal of Colloid and Interface Science, 1981
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973