FAILURE OF GUINEA PIG ANTIBODY TO BEEF INSULIN TO NEUTRALIZE PANCREATIC AND SERUM GUINEA PIG INSULIN ACTIVITY IN VITRO

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Abstract
In vitro studies of the ability of sera of guinea pigs containing antibodies to beef insulin to neutralize guinea pig insulin extracted from serum and pancreas were carried out. Glycogen synthesis by mouse hemidiaphragm was used as the biologic indicator of insulin activity. The sera were incapable of neutralizing the biologic activity of any of the samples of guinea pig insulin tested. This was true despite the fact that the antibody content of the serum was greatly in excess of the content required to neutralize a beef insulin standard completely. Some of the possible reasons for divergent findings of different investigators who have studied the problem of guinea pig insulin neutralization by sera of guinea pigs containing antibodies to beef insulin are discussed.

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