EFFECT OF INJECTION MEDIUM ON QUANTAL ASSAY OF CHORIONIC GONADOTROPHIN IN MALE TOADS
- 1 February 1957
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 24 (2) , 113-118
- https://doi.org/10.1530/acta.0.0240113
Abstract
The comparative biological activity of human chorionic gonadotropin (HCG) when dissolved in saline, human serum, albumin, gamma-globulin, and Plasmosan was studied. Those solvents characterized by large molecular size increased the biological activity, as evidenced by discharge of spermatozoa, 1.5-1.7 times as compared with saline. The authors suggest that this increase in activity is due to the formation of a colloidal coating around the active principle. This coating, they postulate, decreases the rate at which HCG is destroyed after injection. Neither corticotropin nor cortisone when added to HCG in saline solution altered the biological activity.Keywords
This publication has 3 references indexed in Scilit:
- SOURCES OF ERROR IN CLINICAL BIOASSAYS OF SERUM CHORIONIC GONADOTROPINJournal of Clinical Endocrinology & Metabolism, 1955
- Augmentation of Human Chorionic Gonadotrophin by Plasma and Other Non-Hormonal Substances.Experimental Biology and Medicine, 1953
- A CLINICAL BIO-ASSAY FOR CHORIONIC GONADOTROPINJournal of Clinical Endocrinology & Metabolism, 1951