Scission of RNA by the chemical nuclease of 1,10-phenanthroline-eopper ion: preference for single-stranded loops
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (13) , 5361-5376
- https://doi.org/10.1093/nar/17.13.5361
Abstract
The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAphe as substrates. Since the chemical mechanism of scission involves oxidative attack on the ribose, scission is observed at all nucleotides including dihydrouridine and Y-bases. Specificity for single-stranded loop regions is apparent from the similarity of the reactivity of OP-Cu to the single-strand specific reagents dimethyl sulfate and diethyl pyrocarbonate using the fragment of lac mRNA as a substrate. Similar preference is observed in the reaction with tRNA although scission in the helical acceptor stem is also observed.Keywords
This publication has 13 references indexed in Scilit:
- Effects of single-base bulges on intercalator binding to small RNA and DNA hairpins and a ribosomal RNA fragmentBiochemistry, 1989
- Secondary structure of the circular form of the Tetrahymena rRNA intervening sequence: a technique for RNA structure analysis using chemical probes and reverse transcriptase.Proceedings of the National Academy of Sciences, 1985
- Conformational analysis of lac promoters using the nuclease activity of 1,10‐phenanthroline‐copper ionBiopolymers, 1985
- RNA structure analysis using methidiumpropyl-EDTA.Fe(II): a base-pair-specific RNA structure probe.Proceedings of the National Academy of Sciences, 1984
- Structural specificities of five commonly used DNA nucleasesJournal of Molecular Biology, 1984
- Cloning and expression of the gene for bacteriophage T7 RNA polymerase.Proceedings of the National Academy of Sciences, 1984
- Optimal computer folding of large RNA sequences using thermodynamics and auxiliary informationNucleic Acids Research, 1981
- Oxygen-dependent cleavage of DNA by the 1,10-phenanthroline . cuprous complex. Inhibition of Escherichia coli DNA polymerase I.Journal of Biological Chemistry, 1979
- Direct chemical method for sequencing RNA.Proceedings of the National Academy of Sciences, 1979
- Crystal structure of yeast phenylalanine transfer RNAJournal of Molecular Biology, 1978