Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry
- 1 September 1984
- Vol. 5 (5) , 547-549
- https://doi.org/10.1002/cyto.990050519
Abstract
A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro.Keywords
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