Properties of a Brain Adenosine Triphosphatase Sensitive to DDT12
- 1 October 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Economic Entomology
- Vol. 65 (5) , 1238-1245
- https://doi.org/10.1093/jee/65.5.1238
Abstract
Adenosine triphosphatase (ATP phosphohydrolase, E. C. 3.6.1.3) activity which is sensitive to DDT as compared with a noninsecticidal analogue DDE has been found in the synapses of rat brain. Inhibition of the Mg+-, Na+-, and K+-stimuated enzyme component by DDT required a particular combination of the cations which did not favor optimal Na+–K+ ATPase activity. The system exhibited a negative temperature correlation in DDT sensitivity. The I50 of the Na+ and K+ activated component against DDT was 10-6 M, and the system was relatively insensitive to other chlorinated hydrocarbon insecticides. A portion of the enzyme activity could be “solubilized” by ultrasonication and eluted as one peak on Sephadex columns together with bound 14C-DDT.Keywords
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