Multiple Forms of Bacterial NADP-Specific Isocitrate Dehydrogenase

Abstract
Electrophoretically distinct forms of nicotinamide adenine dinucleotide phosphate-specific isocritrate dehydrogenase have been observed in extracts of Escherichia coli grown under different culture conditions. In glucose-grown cells, two distinct bands of isocitrate dehydrogenase activity were observed on polyacrylamide gels and have been completely resolved by employing ion-exchange chromatography. These multiple forms of the enzyme have been studied and their possible metabolic role is discussed.

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