Characterization of Brucella suis clpB and clpAB Mutants and Participation of the Genes in Stress Responses

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Abstract
Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis , a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis , and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress response proteins. Under high-temperature stress conditions, ClpB of B. suis was induced, and an isogenic B. suis clpB mutant showed increased sensitivity to high temperature, but also to ethanol stress and acid pH. The effects were reversible by complementation. Simultaneous inactivation of clpA and clpB resulted in a mutant that was sensitive to oxidative stress. In B. suis expressing gfp , ClpA but not ClpB participated in degradation of the green fluorescent protein at 42°C. We concluded that ClpB was responsible for tolerance to several stresses and that the lethality caused by harsh environmental conditions may have similar molecular origins.

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