Role of DNA Repair by Nonhomologous-End Joining in Bacillus subtilis Spore Resistance to Extreme Dryness, Mono- and Polychromatic UV, and Ionizing Radiation
Open Access
- 15 April 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (8) , 3306-3311
- https://doi.org/10.1128/jb.00018-07
Abstract
The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants ( recA , splB , ykoU , ykoV , and ykoU ykoV mutants) of Bacillus subtilis . NHEJ-defective spores with mutations in ykoU , ykoV , and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than wild-type spores, indicating that NHEJ provides an important pathway during spore germination for repair of DNA double-strand breaks.This publication has 65 references indexed in Scilit:
- Testing interplanetary transfer of bacteria between Earth and Mars as a result of natural impact phenomena and human spaceflight activitiesActa Astronautica, 2007
- The Forespore Line of Gene Expression in Bacillus subtilisJournal of Molecular Biology, 2006
- Making Ends Meet: Repairing Breaks in Bacterial DNA by Non-Homologous End-JoiningPLoS Genetics, 2006
- The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflightMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2005
- Non-homologous end-joining: bacteria join the chromosome breakdanceTrends in Biochemical Sciences, 2003
- A family of DNA repair ligases in bacteria?FEBS Letters, 2001
- Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amberScience, 1995
- DNA-strand breaks limit survival in extreme drynessDiscover Life, 1991
- Mutation induction in spores ofBacillus subtilis by accelerated very heavy ionsRadiation and Environmental Biophysics, 1986
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958