STUDIES ON MEMBRANE-BOUND RECEPTORS FOR ANTIGEN

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Abstract
The effect of polyadenylic: polyundylic acid complexes (poly A:U) on the amount of antibody on the surface of various populations of mouse lymphoid cells has been investigated by means of a sensitive measure of such activity—the binding by primed cell populations of ß-galactosidase (ßGZ) as an antigen. The sensitivity derives from the liberation of fluorescein from an artificial substrate, fluorescein-di-ß-galactopyranoside (FDßG). After incubation with 100 ng/ml of poly A:U, only 40% of the cells previously showing antigen-binding were still active. The optimum range of activity lay between 0.01–1.0 µg/ml poly A:U. Such cells showed increased RNA and protein synthesis as indicated by [3H]uridine and [14C]amino acid incorporation. The polynucleotide effect was abolished by incubation of the cells with sodium azide or iodoacetate, but not by puromycin. When the proteins on the cell surface were labeled by 125I, poly A:U caused their release into the medium.

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