Detection of RNA-Instructed DNA Polymerase and High Molecular Weight RNA in Malignant Tissue
- 1 August 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (8) , 2020-2024
- https://doi.org/10.1073/pnas.69.8.2020
Abstract
An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary tumor virus by molecular hybridization, thus, completing the proof that an RNA-instructed DNA polymerase has been identified. Further, RNA-instructed DNA polymerase and its 70S RNA template are physically associated in a particle that has a density characteristic of oncornaviruses. These experiments now provide the technology required to perform similar examinations of human neoplasias.Keywords
This publication has 8 references indexed in Scilit:
- Viral-Related RNA in Hodgkins' Disease and Other Human LymphomasProceedings of the National Academy of Sciences, 1972
- Evidence for Translation of Viral-Specific RNA in Cells of a Mouse Mammary CarcinomaProceedings of the National Academy of Sciences, 1972
- Detection of High-Molecular-Weight RNA in Particles from Human MilkScience, 1972
- RNA in Human Leukemic Cells Related to the RNA of a Mouse Leukemia VirusProceedings of the National Academy of Sciences, 1972
- Human Sarcomas Contain RNA Related to the RNA of a Mouse Leukemia VirusScience, 1972
- Presence in Human Breast Cancer of RNA homologous to Mouse Mammary Tumour Virus RNANature, 1972
- Simultaneous Detection of Reverse Transcriptase and High Molecular Weight RNA Unique to Oncogenic RNA VirusesScience, 1971
- Characterization of the Products of RNA-directed DNA Polymerases in Oncogenic RNA VirusesNature, 1970