Effects of Digestion and Refrigeration on the Ability of Trichinella spiralis to Infect Rats

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Abstract
Trichina-infected rat meat was artificially digested for 4, 8 and 12 hrs., respectively. The larvae obtained from each period of digestion were subjected to 0, 24, 48 and 72 hrs. of refrigeration at 5 C before being used for infecting rats with 3000 larvae each. The rats were killed after 24 hrs. The number of trichina larvae recovered from the intestine of each rat indicated that 4 and 8 hrs. of digestion and 24 hrs. storage in the refrigerator did not impair the infectivity of the larvae. A 12-hr. period of digestion resulted in a lowering of the number of worms recovered. Exposure to 72 hrs. of refrigeration was injurious to the larvae even in combination with few hrs. of digestion. The entire procedure was repeated with 5000-larvae doses.

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