Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic technique
- 1 February 1993
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 90 (6) , 655-656
- https://doi.org/10.1007/bf00202486
Abstract
A polymorphic microsatellite (Y-27H39) based on a (GATA) n repeat was recently discovered on the short arm of the human Y chromosome. We have used a simple technique based on polymerase chain reaction amplification and native polyacrylamide gel electrophoresis followed by highly sensitive silver staining to study the inheritance, the genetic stability and the allele frequency distribution of this polymorphism in the Brazilian population. We have analyzed 100 randomly chosen Caucasian Brazilian father-son pairs with established paternity. Five alleles, four base-pairs apart, were easily distinguishable. Their frequencies were: A (186 bp), 0.19; B (190 bp), 0.49; C (194 bp), 0.24; D (198 bp), 0.07; E (202 bp), 0.01. In all father-son pairs, there was complete allelic concordance. From these data, the probability of discrimination for forensic cases and the average probability of exclusion for paternity cases were both calculated to be 0.66.Keywords
This publication has 12 references indexed in Scilit:
- Simple repeat sequences on the human Y chromosome are equally polymorphic as their autosomal counterpartsHuman Genetics, 1992
- Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case workForensic Science International, 1992
- DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probesElectrophoresis, 1991
- The human Y chromosome shows a low level of DNA polymorphismAnnals of Human Genetics, 1990
- Y chromosome DNA haplotyping suggests that most European and Asian men are descended from one of two malesGenomics, 1990
- F10, the gene for the glycine-rich major eggshell protein ofSchistosoma mansonirecognizes a family of hypervariable minisatellites in the human genomeNucleic Acids Research, 1990
- A search for restriction fragment length polymorphism on the human Y chromosomeHuman Genetics, 1989
- Optimized oligonucleotide probes for DNA fingerprintingElectrophoresis, 1988
- Clustered GATA repeats (Bkm sequences) on the human Y chromosomeHuman Genetics, 1986
- Paternity testing with genetic markers: Are Y‐linked genes more efficient than autosomal ones?American Journal of Medical Genetics, 1985