Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells
- 1 October 1990
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 18 (3) , 265-267
- https://doi.org/10.1007/bf00318391
Abstract
Summary The cationic lipophilic dye Rhodamine 123 (Rh123) is selectively enriched in mitochondria in a membrane potential-dependent manner. Application of drugs which interfere with the electron flow of the respiratory chain lead to a severe reduction of mitochondrial dye uptake. In this communication we show that the same effect is observed after Rh123-staining of respiratory-deficient yeast mutants. Based on this observation we used flow cytometry to discriminate respiratory-compentent and respiratory-deficient yeast cells. Combined with a cell sorter we were able to selectively enrich respiring and non-respiring yeast cells, repectively, from a mixture of cells.Keywords
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