A new blotting medium for the simple isolation and Identification of highly resolved messenger RNA
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (3) , 1349-1360
- https://doi.org/10.1093/nar/12.3.1349
Abstract
A simple method has been developed that allows the rapid isolation and identification of highly resolved mRNA molecules. RNA species are separated by gel electrophoresis and then blotted on to a paper sheet to which polyuridylic acid has been covalently bound. This mRNA affinity paper ("mAP") specifically binds, in a reversible manner, polyA+ containing molecules. A replica picture of the agarose gel is thus obtained on the mAP, from which bound mRNA molecules can be eluted by heating in water. In addition to their simple isolation individual mRNA species, whilst still bound to mAP, can be identified by both "in-situ" hybridization and translation.Keywords
This publication has 14 references indexed in Scilit:
- The structure and expression of the preproenkephalin geneNucleic Acids Research, 1982
- Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.Proceedings of the National Academy of Sciences, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Ribosomal RNA cleavage, nuclease activation and 2-5A (ppp(A2′p)nA) in interferon-treated cellsNucleic Acids Research, 1981
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Interaction between Membrane Functions and Protein Synthesis in ReticulocytesEuropean Journal of Biochemistry, 1978
- Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proceedings of the National Academy of Sciences, 1977
- Mapping adenines, guanines, and pyrimidines in RNANucleic Acids Research, 1977
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- The genes for silk fibroin in Bombyx moriJournal of Molecular Biology, 1972