Construction of a Reading Frame–Independent Yeast Two-Hybrid Vector System for Site-Specific Recombinational Cloning and Protein Interaction Screening
Open Access
- 1 September 2008
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 45 (3) , 235-244
- https://doi.org/10.2144/000112897
Abstract
The yeast two-hybrid (Y2H) system is a powerful method to identify protein-protein inter-actions (PPI) in vivo, requiring minimal prior information of the putative interactors. The time and effort required for each experiment can...Keywords
This publication has 11 references indexed in Scilit:
- A genome-wide study of dual coding regions in human alternatively spliced genesGenome Research, 2005
- The Evolution of Transcription-Initiation SitesMolecular Biology and Evolution, 2005
- ORFeome Cloning and Systems Biology: Standardized Mass Production of the Parts From the Parts-ListGenome Research, 2004
- DNA Cloning Using In Vitro Site-Specific RecombinationGenome Research, 2000
- [34] GATEWAY recombinational cloning: Application to the cloning of large numbers of open reading frames or ORFeomesPublished by Elsevier ,2000
- The F Plasmid CcdB Protein Induces Efficient ATP-dependent DNA Cleavage by GyraseJournal of Molecular Biology, 1993
- Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two‐hybrid systemThe FASEB Journal, 1993
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Fluorescence detection in automated DNA sequence analysisNature, 1986
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977