Interaction between erythroblasts and macrophages in vitro: effect of neuraminidase-treatment of erythroblasts and the role of serum factors

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Abstract
Some factors influencing the interaction in vitro between dimethylsulfoxide-induced Friend leukemia erythroblasts (IFLE) and syngeneic mouse peritoneal macrophages (Mo) were investigated. Desialation of erythroblasts by treatment with neuraminidase resulted in a significant increase in their association with and ingestion by Mo. In addition, the interaction with Mo of both neuraminidase-treated and untreated IFLE was found to be influenced by a heat-stable serum factor. .gamma.-Globulin markedly enhanced the weak IFLE-Mo interaction which occurred at low serum concentration suggesting that the heat-stable factor may be an Ig. Desialation of the IFLE-rendered them agglutinable by peanut lectin which was used as a probe for neuraminidase-induced membrane changes. By contrast, cycloheximide treatment of IFLE which also enhances their interaction with Mo, did not result in the exposure of receptors for this lectin. Both desialation and cycloheximide treatment of IFLE apparently lead to secondary alterations in their membrane structure which renders them recognizable by Mo.