The Biological Assay of Vegetable Purgatives
- 1 September 1949
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 1 (1) , 673-682
- https://doi.org/10.1111/j.2042-7158.1949.tb12480.x
Abstract
Summary: A method for the biological assay of vegetable purgatives based on the number of wet fæces produced by groups of mice after dosing is described. The relation of the number of wet fæces per kg. of mouse to the logarithm of the dose was found to be linear. 40 mice divided equally into 4 groups were used in each assay.*** 2 groups received the standard preparation and the other 2 groups received the test preparation. The standard deviation of a single determination based on 9 such assays was estimated to be 15ṁ7 per cent. The limits of error (P = 0ṁ99) for a single assay are 100±40ṁ5 per cent. A special cage has been designed for this assay, and it has been found advantageous to incorporate a definite proportion of water in the diet, during test. The method described is not only convenient in use but also gives a comparatively high degree of accuracy. The method has been successfully applied to senna leaf, senna fruit and extracts and commercial preparations made from these drugs, pure glycosides (sennosides A and B), and pure anthracene compounds (aloe-emodin and aloe-emodin anthranol).Keywords
This publication has 10 references indexed in Scilit:
- Factors influencing the cathartic activity of senna in mice**Acknowledgment is made of a grant from the Centaur Company and the Chattanooga Medicine Company in support of this study.Journal of the American Pharmaceutical Association (Scientific ed.), 1945
- The White Rat in the Biological Assay of Senna and Its PreparationsJournal of the American Pharmaceutical Association (Scientific ed.), 1945
- Laxative action in mice of tinnevelly and alexandria senna, and of several botanically related plants**From the Research Laboratory of The Chattanooga Medicine Co., Chattanooga, Tenn.Journal of the American Pharmaceutical Association (Scientific ed.), 1944
- Further Studies on Cathartic Action in Mice: Senna, Aloe, Cascara and Bile Salts**Received from the Henry E. Kalusowski Memorial Laboratory, George Washington University School of Pharmacy, Washington, D. C.Journal of the American Pharmaceutical Association (Scientific ed.), 1944
- A Study of the Precipitation in Fluidextract of Senna**Presented to the Scientific Section of the A. Ph. A., Denver meeting, 1942.Journal of the American Pharmaceutical Association (Scientific ed.), 1943
- An Evaluation of the Cathartic Action of Bile Salts Following Acute and Chronic Administration to Mice*Journal of the American Pharmaceutical Association (Scientific ed.), 1942
- Bioassay of Senna Leaves and of the Fluidextract of Senna, U. S. P. XIJournal of the American Pharmaceutical Association (Scientific ed.), 1940
- Evaluation of Cathartics**Presented before the Scientific Section, A. Ph. A., New York meeting, 1937, and illutrated with Micro-Movies.††From the Gross laboratory for Biological and Biochemical Research.The Journal of the American Pharmaceutical Association (1912), 1938
- Über die wirksamen Inhaltsstoffe der Folia SennaeNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1936
- The Constituents in Cascara Sagrada Extract. 2. A Method of Bioassay**The authors are indebted to the Parke, Davis and Company for a research grant (for M. W. G.) and for supplying the cascara bark.,11Contribution No. 307 from the Department of Chemistry, University of Pittsburgh.The Journal of the American Pharmaceutical Association (1912), 1936