Differential response of the human 6–16 and 9–27 genes to α and γ interferons
- 1 January 1991
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (3) , 591-598
- https://doi.org/10.1093/nar/19.3.591
Abstract
9-27 mRNA is expressed to a high level in response to both alpha and gamma interferons. In contrast, 6-16 mRNA is expressed well in response to alpha but very poorly in response to gamma interferon in human cells. The factors governing these different levels of expression were investigated. For both genes the major effect of both interferons is on transcription. A transcriptional bias in the 6-16 promoter/enhancer accounts in large part for the differential response of 6-16 to the two interferons. No single DNA element appears responsible; the smaller the 5' region analysed the lower the absolute activity and the smaller the differential response to alpha and gamma interferons observed. Both the 6-16 and 9-27 mRNAs are very stable and no effect of the interferons on stability was detected. Nor was any direct evidence obtained for preferential processing of the 9-27 mRNA. Nevertheless, differentials between the transcription and accumulation of mature mRNAs, particularly for 6-16 mRNA in response to gamma interferon, suggest that post-transcriptional control(s) must additionally operate. The 9-27 5' promoter/enhancer is much less active than that from 6-16 when placed 5' of a marker gene, despite the similar response of the two genes to alpha interferon.Keywords
This publication has 18 references indexed in Scilit:
- The “initiator” as a transcription control elementCell, 1989
- Rapid activation by interferon alpha of a latent DNA-binding protein present in the cytoplasm of untreated cells.Proceedings of the National Academy of Sciences, 1989
- Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter.Molecular and Cellular Biology, 1988
- Genomic characterization of a gamma-interferon-inducible gene (IP-10) and identification of an interferon-inducible hypersensitive site.Molecular and Cellular Biology, 1987
- Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.Proceedings of the National Academy of Sciences, 1987
- CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elementsNucleic Acids Research, 1987
- Regulation of a transfected human class II major histocompatibility complex gene in human fibroblasts.Proceedings of the National Academy of Sciences, 1986
- Nonidentical Induction of the Guanylate Binding Protein and the 56K Protein by Type I and Type II InterferonsJournal of Interferon Research, 1986
- 3′ End structure of the human (2′–5′) oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expressionNucleic Acids Research, 1985
- Analysis and Purification of Human Lymphoblastoid (Namalwa) Interferon Using a Monoclonal AntibodyJournal of General Virology, 1982