IMMUNOCHEMICAL STUDY OF CANINE INTESTINAL, HEPATIC, AND OSSEOUS ALKALINE-PHOSPHATASES

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (9) , 1506-1509
Abstract
Partially purified alkaline phosphatase [EC 3.1.3.1] (ALP) from canine intestine, liver and bone were injected into rabbits to elicit anti-canine intestinal, hepatic and osseous ALP antibodies, respectively. The antibody formed a soluble enzyme-antienzyme complex when directly interacted with the ALP antigen. In order to form an insoluble complex, it was then necessary to interact the initial soluble complex with the goat anti-rabbit .gamma.-globulin antibody to the 2nd step. Anti-intestinal ALP antibody was highly specific and did not cross react with canine hepatic, osseous, splenic and renal ALP. Antiliver and antibone ALP antibodies did cross react with hepatic, osseous, splenic and renal ALP, but not with the intestinal ALP.

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