Metabolic suppressors of trimethoprim and ultraviolet light sensitivities of Saccharomyces cerevisiae rad6 mutants
- 1 September 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 139 (3) , 866-876
- https://doi.org/10.1128/jb.139.3.866-876.1979
Abstract
Dominant mutations at 2 newly identified loci, designated SRS1 and SRS2, that metabolically suppress the trimethoprim sensitivity of rad6 and rad18 strains, were isolated from trimethoprim-resistant mutants arising spontaneously in rad6-1 rad18-2 strains of the yeast S. cerevisiae. The SRS2 mutations also efficiently suppress the UV light sensitivity of the parent strains. They do not suppress their sensitivity to ionizing radiation or their deficiency with respect to induced mutagenesis and sporulation. Such observations support the hypothesis that RAD6-dependent activities can be separated into two functionally distinct groups: a group of error-free repair activities that are responsible for a large amount of the radiation resistance of wild-type strains and also for their resistance to trimethoprim, and a group of error-prone activities that are responsible for induced mutagenesis and are also important in sporulation, but which account at best for only a very small amount of wild-type recovery.This publication has 21 references indexed in Scilit:
- Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeastJournal of Molecular Biology, 1978
- Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiaeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Alkaline sucrose sedimentation studies of MMS-induced DNA single-strand breakage and rejoining in the wild type and in UV-sensitive mutants of Saccharomyces cerevisiaeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Spontaneous mutation by mutagenic repair of spontaneous lesions in DNANature, 1976
- Yeast mutants sensitive to trimethoprimMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- A genetic study of X-ray sensitive mutants in yeastMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Repair systems in SaccharomycesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeastJournal of Molecular Biology, 1974
- The genetic control of dark recombination in yeastMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1971
- The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeastMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1968