SPECIFIC UPTAKE OF HEYMANNS NEPHRITIC KIDNEY ELUATE BY RAT-KIDNEY - STUDIES INVIVO AND IN ISOLATED PERFUSED KIDNEYS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 46  (4) , 450-453
Abstract
Antiglomerular antibodies are found in Ig eluted from kidneys of rats with Heymann''s nephritis. To demonstrate a role for these antibodies in the pathogenesis of Heymann''s nephritis, paired-label radioisotope studies were used to quantitate binding of the eluted antibody to glomeruli of isolated perfused rat kidneys. In this perfusion situation, which largely excludes the formation of circulation immune complexes, specific binding of 0.9-3.2% of the total eluate protein infused was found with 9-33% of the bound protein recovered in the glomerular fractions. Specific glomerular binding was observed after administration of the paired-label mixture to intact rats. Glomerular Ig deposits and subepithelial electron-dense deposits similar to those found in rats with Heymann''s nephritis were produced by i.v. administration of the eluted antibody. Direct binding of antibody to glomerular capillary wall antigens, in a manner similar to that established for antiglomerular basement membrane antibody, must be considered in the immunopathogenesis of Heymann''s nephritis and potentially in some forms of human glomerular injury.