Autoradiography of Ganglioside Antigens Separated by High-Performance. Thin-Layer Chromatography vith Their Antibodies1
- 1 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 96 (1) , 261-264
- https://doi.org/10.1093/oxfordjournals.jbchem.a134821
Abstract
An improved method is described for the detection of ganglioside antigens with the antibodies on tbin-layer.chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chrornatographic plate, application of antibody solution, and detection of bound antibody with [ 125 ]staphy-lococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an ant i-glycolipid antibody.Keywords
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