Autonomous Detection of Aerosolized Biological Agents by Multiplexed Immunoassay with Polymerase Chain Reaction Confirmation
- 2 December 2004
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 77 (1) , 284-289
- https://doi.org/10.1021/ac0489014
Abstract
The autonomous pathogen detection system (APDS) is an automated, podium-sized instrument that continuously monitors the air for biological threat agents (bacteria, viruses, and toxins). The system has been developed to warn of a biological attack in critical or high-traffic facilities and at special events. The APDS performs continuous aerosol collection, sample preparation, and detection using multiplexed immunoassay followed by confirmatory PCR using real-time TaqMan assays. We have integrated completely reusable flow-through devices that perform DNA extraction and PCR amplification. The fully integrated system was challenged with aerosolized Bacillus anthracis, Yersinia pestis, Bacillus globigii, and botulinum toxoid. By coupling highly selective antibody- and DNA-based assays, the probability of an APDS reporting a false positive is extremely low.Keywords
This publication has 4 references indexed in Scilit:
- Development of an Automated Sample Preparation Module for Environmental Monitoring of Biowarfare AgentsAnalytical Chemistry, 2004
- Technology Challenges in Responding to Biological or Chemical Attacks in the Civilian SectorScience, 2003
- A Reusable Flow-Through Polymerase Chain Reaction Instrument for the Continuous Monitoring of Infectious Biological AgentsAnalytical Chemistry, 2003
- Gene Walking by PCR Amplification of Short Fragments from Taq DNA Polymerase—Modified P1 Plasmid DNA and TA CloningBioTechniques, 1999