Identification of an Arabidopsis thaliana Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase (RCA) Minimal Promoter Regulated by Light and the Circadian Clock
Open Access
- 1 September 1996
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 112 (1) , 43-51
- https://doi.org/10.1104/pp.112.1.43
Abstract
Transcription of the Arabidopsis thaliana gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is organ-specific, light-responsive, and regulated by the circadian clock. RCA is transcribed throughout the green parts of the plant, but not in roots and petals. Responses elicited by short pulses of light indicate that the light response is mediated, at least in part, by phytochrome. Analysis of transgenic tobacco and Arabidopsis carrying RCA 5[prime] untranscribed regions fused to reporter genes (uidA, encoding [beta]-glucuronidase, or cat, encoding chloramphenicol acetyltransferase) indicate that elements sufficient to confer organ-specific, light-responsive, and clock-regulated transcription are localized within 317 base pairs upstream of the site of transcription initiation. A clock-responsive element sufficient to confer a low-amplitude (approximately 2-fold) circadian oscillation lies within 317 base pairs of the transcription start, but other elements necessary for high-amplitude (approximately 10-fold) circadian oscillation lie upstream of -317 and are removed by deletion from -970 to -317.Keywords
This publication has 24 references indexed in Scilit:
- A DNA binding activity for one of two closely defined phytochrome regulatory elements in an Lhcb promoter is more abundant in etiolated than in green plants.Plant Cell, 1996
- Multiple DNA-Protein Complexes at a Circadian-Regulated Promoter Element.Plant Cell, 1995
- PHYTOCHROME PHOTOTRANSDUCTION PATHWAYSAnnual Review of Genetics, 1994
- Circadian clock‐ and phytochrome‐regulated transcription is conferred by a 78 bp cis‐acting domain of the Arabidopsis CAB2 promoterThe Plant Journal, 1994
- Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter.Plant Cell, 1994
- Constitutive, light‐responsive and circadian clock‐responsive factors compete for the different I box elements in plant light‐regulated promotersThe Plant Journal, 1993
- CA-1, a novel phosphoprotein, interacts with the promoter of the cab140 gene in Arabidopsis and is undetectable in det1 mutant seedlings.Plant Cell, 1993
- Molecular light switches for plant genes.Plant Cell, 1990
- Coordinate Expression of Rubisco Activase and Rubisco during Barley Leaf Cell DevelopmentPlant Physiology, 1989
- A Mutant of Arabidopsis thaliana Which Lacks Activation of RuBP Carboxylase In VivoPlant Physiology, 1982