Abstract
SUMMARY: The nutrition of the free‐living phase of Conchocelis of P. tenera was studied axenically. Conch‐ocelis preferred NO3, as nitrogen source. Urea and NH4 in low concentration, asparagine, and lysine were very good N sources. Several other amino acids were also utilized but growth was less abundant. Inorganic and organic phosphates were utilized; they were required at relatively low concentrations. Glycerophosphate gave excellent growth in a comparatively wide range of concentrations (0.1‐5 mg P %). The optimal Ca concentration was 10‐100 mg %. Needs for boron, manganese, zinc, strontium, rubidium, lithium, and iodine were demonstrated. The iodine effect was remarkable (peak growth with 1μg %); the effective concentration range was very narrow. Iron, cobalt, and bromine seemed to be adequately supplied as impurities of the macro‐nutrients. A modified artificial medium (ASP12I) for the Conchocelis phase is presented.