8‐Hydroxydeoxyguanosine

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Abstract
8‐Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 8OHDG by HPLC. Further analysis by GC‐MS confirmed that levels of 8OHDG in SLE urine were 103‐fold lower than in an age‐ and sex‐matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 8OHDG. We subsequently observed in SLE patients that 8OHDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.