Incidence of Metabolic Changes among Virus-Resistant Mutants of a Bacterial Strain

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Abstract
By subjecting the virus-sensitive strain Escherichia coli B to the action of 3 strains of bacterial virus it was possible to isolate in pure culture 57 mutants of E. coli which differ from the original strain by their resistance to 1, 2 or all 3 viruses in all 7 possible combinations. All are similar to the parent organism, being gram-negative, non-motile rods. 28 were unable to grow in a basal medium adequate for the parent strain, but were able to grow when 0.005% yeast extract was added. Supplements of 3 vits., 23 amino acids and hydrolized casein were tried on 3 mutants but none of these, added singly or in combination, supported growth. The mutation which renders the strain virus-resistant (through affecting the synthesis of a constituent essential to the reaction of cell and virus or the capacity to absorb virus) may in some cases require factors for growth beyond those needed by the organism from which it arose.

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