Use of PCR with feces for detection of Helicobacter pylori infections in patients
- 1 May 1994
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 32 (5) , 1346-8
- https://doi.org/10.1128/jcm.32.5.1346-1348.1994
Abstract
PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during 2 weeks with omeprazole (40 mg each day) to increase gastric pH, feces were examined as well. H. pylori target DNA could not be detected in the stools of any of the 24 infected patients. It was concluded that there was no substantial shedding of H. pylori in feces from either group of patients.Keywords
This publication has 16 references indexed in Scilit:
- Transmission of Helicobacter pylori via faecesThe Lancet, 1993
- PCR identification of Helicobacter pylori in faeces from gastritis patientsThe Lancet, 1993
- Helicobacter (aka Campylobacter) pylori as the major causal factor in chronic hypochlorhydriaMedical Hypotheses, 1992
- Molecular biology and infections of the gut.Gut, 1992
- Isolation of Helicobacter pylori from human faecesThe Lancet, 1992
- Epidemiology of Helicobacter pylori in Southern China: Identification of Early Childhood as the Critical Period for AcquisitionThe Journal of Infectious Diseases, 1992
- Transmission ofHelicobacterspp. A challenge to the dogma of faecal-oral spreadEpidemiology and Infection, 1991
- Water source as risk factor for Helicobacter pylori infection in Peruvian childrenThe Lancet, 1991
- Seroprevalence of Helicobacter pylori Infections in ThailandThe Journal of Infectious Diseases, 1990
- INGESTION OF CAMPYLOBACTER-PYLORIDIS CAUSES GASTRITIS AND RAISED FASTING GASTRIC PH1987