A genome-scale shRNA resource for transgenic RNAi in Drosophila
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- 3 April 2011
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 8 (5) , 405-407
- https://doi.org/10.1038/nmeth.1592
Abstract
Short hairpin RNAs, expressed from microRNA scaffold–containing vectors, efficiently silence gene expression in female germ cells as well as somatic cells in the fly. A genome-wide resource is being developed. Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.Keywords
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