Different rate-limiting steps in excision repair of ultraviolet- and N-acetoxy-2-acetylaminofluorene-damaged DNA in normal human fibroblasts.
Open Access
- 1 April 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (4) , 1548-1552
- https://doi.org/10.1073/pnas.74.4.1548
Abstract
In normal human [fibroblast Rid Mor CRL 1220] cells the amount of excision of UV damage to DNA saturates at high doses. In these cells from chemicals mimic UV damage as far as their biological and repair characteristics are concerned. One of these chemicals is N-acetoxy-2-acetylaminofluorene. The limited repair capacity for UV damage was determined. The effect of treatment with N-acetoxy-2-acetylaminofluorene on the limited repair capacity for UV damage was determined. To measure repair unscheduled DNA synthesis and the number of sites sensitive to an UV endonuclease were determined in an assay using an extract of Micrococcus luteus. The nuclease does not act on DNA treated with the chemical. The amount of unscheduled DNA synthesis due to a combined chemical and UV treatment was the sum of those observed from the separate treatments, even at saturation doses. The combined treatment did not affect the removal of nuclease-sensitive sites. There are probably different rate-limiting steps in excision repair of the UV and the chemical damage. A model involving a complex of enzymes to explain the data is suggested.This publication has 21 references indexed in Scilit:
- Differences in removal of acetylaminofluorene and pyrimidine dimers from the DNA of cultured mammalian cells.Proceedings of the National Academy of Sciences, 1977
- DNA single-strand breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum.Proceedings of the National Academy of Sciences, 1976
- Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus).Proceedings of the National Academy of Sciences, 1975
- Enzymatic Repair of DNAAnnual Review of Biochemistry, 1975
- Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.1974
- A sensitive method to measure physical and chemical carcinogen-induced “unscheduled DNA synthesis” in rapidly dividing eukaryotic cellsExperimental Cell Research, 1974
- Use of a UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Effects of inhibitors on repair of DNA in normal human and xeroderma pigmentosum cells after exposure to X-rays and ultraviolet irradiationMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- DNA repair with purines and pyrimidines in radiation- and carcinogen-damaged normal and xeroderma pigmentosum human cells.1973
- Effects of Radiation on PolynucleotidesAnnual Review of Biophysics and Bioengineering, 1972