Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture
- 1 March 1991
- journal article
- biotechnology
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 34 (6) , 735-741
- https://doi.org/10.1007/bf00169343
Abstract
Summary Diethylaminoethyl-derivatized dextran microspheres were used to cultivate Chinese hamster ovary, 293, Vero and swine testicular cells. Cells became attached to the microspheres but did not spread out. Instead, they grew in a more spherical shape and eventually formed multiple-cell-layer aggregates. Viability in these aggregates remained high after the cultures reached high cell concentrations. This cultivation method allows a high cell density to be achieved with a low microsphere concentration.Keywords
This publication has 13 references indexed in Scilit:
- Improved microscopic observation of mammalian cells on microcarriers by fluorescent stainingCytotechnology, 1991
- Characterization and Novel Purification of Recombinant Human Protein C from Three Mammalian Cell LinesNature Biotechnology, 1990
- Attachment and growth of mammalian cells on microcarriers with different ion exchange capacitiesBiotechnology & Bioengineering, 1987
- The Use of Fluorescein Diacetate and Ethidium Bromide as a Viability Stain for Isolated Islets of LangerhansStain Technology, 1987
- Growth limitations in microcarrier culturesPublished by Springer Nature ,1987
- Serial propagation of mammalian cells on microcarriersBiotechnology & Bioengineering, 1985
- A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriersBiotechnology & Bioengineering, 1985
- Substrate-dependent differences in growth and biological properties of fibroblasts and epithelial cells grown in microcarrier cultureJournal of Biological Standardization, 1985
- Cell aggregate suspension culture for large-scale production of biomoleculesIn Vitro Cellular & Developmental Biology - Plant, 1980
- Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous CultureNature, 1967