The Effect of Proteolytic Enzymes on E. coli Phages and on Native Proteins

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Abstract
Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T2 phage is slowly inactivated by high concentrations of [alpha]-, [beta]-, [gamma]-, or [DELTA]-chymotrypsin, but not by trypsin or ficin. P1 phage is slowly inactivated by [alpha]-, [beta]-, or [DELTA]-chymotrypsin, or ficin, more rapidly by [DELTA]-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystalline serum albumin, E. coli nucleoprotein, and yeast nucleoprotein are hydrolyzed slowly by [alpha]- chymotrypsin. Yeast nucleoprotein, like P1 phage, is hydrolyzed more rapidly by [alpha] -chymotrypsin than by [alpha]-chymotrypsin, but not by trypsin or ficin. Neither phages nor native proteins were attacked by papain, carboxypeptidase, deoxyribonuclease, or ribonuclease.