Neoplastic Transformation In Vivo and In Vitro of Regenerating Hamster Hepatic Cells by Oncogenic DNA Simian Virus 402

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Abstract
Three-week-old male Syrian golden hamsters were partially hepatectomized. At various times after surgery, some of the animals were inoculated intravenously with the oncogenic DNA simian virus 40 (SV40). Other animals were killed; their regenerating livers were excised and grown in culture in the presence of SV40. In both the in vivo and in vitro systems, only hepatic stromal, sinusoidal, and ductal-epithelial cells transformed neoplastically. Hepatic parenchymal cells did not develop oncogenic properties and, as a result, did not induce hepatocellular adenomas or carcinomas. The results indicated that resistance of hepatic parenchymal cells to the oncogenic effect of SV40 persisted, even with stimulation of their proliferative capacity before virus exposure. The significance of these observations was emphasized, particularly in regard to an analogous but reverse phenomenon observed in man, namely, the association of hepatic parenchymal cell hyperplasia with neoplasia.

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