Elg1 forms an alternative RFC complex important for DNA replication and genome integrity
Open Access
- 15 August 2003
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 22 (16) , 4304-4313
- https://doi.org/10.1093/emboj/cdg406
Abstract
Genome‐wide synthetic genetic interaction screens with mutants in the mus81 and mms4 replication fork‐processing genes identified a novel replication factor C (RFC) homolog, Elg1, which forms an alternative RFC complex with Rfc2–5. This complex is distinct from the DNA replication RFC, the DNA damage checkpoint RFC and the sister chromatid cohesion RFC. As expected from its genetic interactions, elg1 mutants are sensitive to DNA damage. Elg1 is redundant with Rad24 in the DNA damage response and contributes to activation of the checkpoint kinase Rad53. We find that elg1 mutants display DNA replication defects and genome instability, including increased recombination and mutation frequencies, and minichromosome maintenance defects. Mutants in elg1 show genetic interactions with pathways required for processing of stalled replication forks, and are defective in recovery from DNA damage during S phase. We propose that Elg1‐RFC functions both in normal DNA replication and in the DNA damage response.Keywords
This publication has 58 references indexed in Scilit:
- Mus81-Eme1 and Rqh1 Involvement in Processing Stalled and Collapsed Replication ForksJournal of Biological Chemistry, 2002
- Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometryNature, 2002
- Functional organization of the yeast proteome by systematic analysis of protein complexesNature, 2002
- The Fuss about Mus81Cell, 2001
- Identification of rad27 Mutations That Confer Differential Defects in Mutation Avoidance, Repeat Tract Instability, and Flap CleavageMolecular and Cellular Biology, 2001
- Rfc4 Interacts with Rpa1 and Is Required for Both DNA Replication and DNA Damage Checkpoints in Saccharomyces cerevisiaeMolecular and Cellular Biology, 2001
- Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymeraseThe EMBO Journal, 1999
- Minichromosome Maintenance as a Genetic Assay for Defects in DNA ReplicationMethods, 1999
- Clamp loading, unloading and intrinsic stability of the PCNA, β and gp45 sliding clamps of human, E. coli and T4 replicasesGenes to Cells, 1996
- Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair.Genes & Development, 1994