An Electron Microscope Study of the Cytophagocytosis of Sarcoma I Cells by Alloimmune Macrophages In Vitro 2

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Abstract
This electron microscope study of cytophagocytosis showed the adherence of Sarcoma I (Sal) cells to alloimmune macrophages, the spread of the macrophage over the surface of the target cell, and the enclosure of the target cell within a phagocytic vacuole. The phagocytized Sal cell was usually tightly enclosed within the phagosome. Stages in the fusion of macrophage cytoplasmic granules with the phagosomal membrane and liberation of granule contents into the phagosome were demonstrated. The accumulation of electronopaque material around the phagocytized cell was attributed to the lysosomal material. A diffuse appearance of the surrounding membranes often accompanied electron-opaque material in the vacuolar space. The effect of this material on the ultrastructure of the phagocytized cell was manifested by multiple invaginations and fusions of the plasma membrane which apparently formed channels from the vacuolar space into the peripheral regions of the cell, and in some instances, appeared to isolate portions of cytoplasm from the cell. These membrane changes probably represented the initial event in the death and digestion of the phagocytized cell.

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