In situ Raman spectroscopic analysis of thin layer chromatograms

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Abstract
Raman spectra of hexamethylenetetramine and several substituted benzophenones have been studied in situ on thin layer chromatography plates. Success depends upon the nature of the substrate (Kieselgel HR was the best found), the eluant, the retention factor, fluorescence of both plate and sample, and the scattering efficiency of the sample itself. It is concluded that the method is viable within the restrictions discussed, but that an instrument with rapid spectrum accumulation facilities would be necessary for routine analytical application.

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