A Green Nonsulfur Bacterium,Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive Organisms

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Abstract
Dehalococcoides ethenogenesis a member of the physiologically diverse division of green nonsulfur bacteria. Using a TBLASTN search, theD. ethenogenes lexAgene has been identified, cloned, and expressed and its protein has been purified. Mobility shift assays revealed that theD. ethenogenesLexA protein specifically binds to both its own promoter and that of theuvrAgene, but not to therecApromoter. Our results demonstrate that theD. ethenogenesLexA binding site is GAACN4GTTC, which is identical to that found in gram-positive bacteria. In agreement with this fact, theBacillus subtilisDinR protein binds specifically to theD. ethenogenesLexA operator. This constitutes the first non-gram-positive bacterium exhibiting a LexA binding site identical to that ofB. subtilis.