Comparative effects of amylin and cholecystokinin on food intake and gastric emptying in rats

Publisher Website
Google Scholar
Abstract
CCK is a physiological inhibitor of gastric emptying and food intake. The pancreatic peptide amylin exerts similar actions, yet its physiological importance is uncertain. Objectives were to compare the dose-dependent effects of intravenous infusion of amylin and CCK-8 on gastric emptying and food intake in rats, and to assess whether physiological doses of amylin are effective. Amylin and CCK-8 inhibited gastric emptying with mean effective doses (ED50s) of 3 and 35 pmol · kg−1 · min−1 and maximal inhibitions of 60 and 65%, respectively. Amylin and CCK-8 inhibited food intake with ED50s of 8 and 14 pmol · kg−1 · min−1 and maximal inhibitions of 78 and 69%, respectively. The minimal effective amylin dose for each effect was 1 pmol · kg−1 · min−1. Our previous work suggests that this dose increases plasma amylin by an amount comparable to that produced by a meal. These results support the hypothesis that amylin acts as a hormonal signal to the brain to inhibit gastric emptying and food intake and that amylin produces satiety in part through inhibition of gastric emptying.