A Synthetic, Partial Pre-mRNA for Ovalbumin Primes Its Own Complementary DNA with Reverse Transcriptase

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Abstract
Synthetic, partial pre-mRNA for ovalbumin was found to prime its own cDNA with reverse transcriptase. Initiation of the cDNA occurred 36 bases upstream of the 3′-end of the RNA, probably as a result of intramolecular base pairing at this end. Inhibition of self-priming occurs following ligation of pCp or poly-adenylation at the 3′-terminus of the synthetic RNA. The secondary structure of 3′-end region of the template RNA strongly affected the ability of self-priming.

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