Cross-Reactive B-Cell Epitopes of Microbial and Human Heat Shock Protein 60/65 in Atherosclerosis
- 1 June 2003
- journal article
- research article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 23 (6) , 1060-1065
- https://doi.org/10.1161/01.atv.0000071701.62486.49
Abstract
Objective— Growing evidence suggests that immune reactions to heat shock protein 60 (HSP60) are involved in atherogenesis. Because of the high phylogenetic conservation between microbial and human HSP60, bacterial infections might be responsible for breaking the tolerance to self-HSP60, which is expressed on the surface of stressed arterial endothelial cells. Methods and Results— We purified serum antibodies to Escherichia coli HSP60 (GroEL), the 60-kD chlamydial HSP, and HSP65 of Mycobacterium tuberculosis by affinity chromatography from clinically healthy subjects with sonographically proven carotid atherosclerosis. Reactivity of the purified antibodies with overlapping human HSP60 peptides was measured, and 8 shared common epitopes, recognized by all anti-bacterial HSP60/65 antibodies, were identified. Antisera specific for these cross-reactive epitopes were produced by immunizing rabbits with peptides derived from human HSP60. By immunohistochemistry, the epitopes were found to be present in the arter...Keywords
This publication has 27 references indexed in Scilit:
- Epitope mapping of heat shock protein 60 (GroEL) fromPorphyromonas gingivalisFEMS Immunology & Medical Microbiology, 2000
- Immunization of Low-density Lipoprotein Receptor Deficient (LDL-RD) Mice with Heat Shock Protein 65 (HSP-65) Promotes Early AtherosclerosisJournal of Autoimmunity, 2000
- Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopesMolecular Immunology, 1999
- Association between antibodies to heat shock protein 65 and coronary atherosclerosis. Possible mechanism of action ofHelicobacter pyloriand other bacterial infections in increasing cardiovascular riskEuropean Heart Journal, 1998
- Macrophage-lysis mediated by autoantibodies to heat shock protein 65/60Atherosclerosis, 1997
- SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modelingElectrophoresis, 1997
- Induction of heat shock protein in monocytic cells by oxidized low density lipoproteinAtherosclerosis, 1996
- Immunoelectron Microscopic Localization of the 60-kDa Heat Shock Chaperonin Protein (Hsp60) in Mammalian CellsExperimental Cell Research, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Surface expression by mononuclear phagocytes of an epitope shared with mycobacterial heat shock protein 60European Journal of Immunology, 1991