Simultaneous Detoxification of Phytoalexins byFusarium solanif. sp.phaseoli

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Abstract
The isoflavonoid phytoalexins, kievitone and phaseollidin, added to liquid cultures of F. solani f. sp. phaseoli [a pathogen on French bean, Phaseolus vulgaris] were simultaneously detoxified to kievitone hydrate and phaseollidin hydrate, respectively. Both phytoalexins (each at 20 .mu.g/ml) were transformed within 24 h after being added to fungal cultures. No increase in mycelial dry weight of F. solani f. sp. phaseoli occurred while detoxification was proceeding. A purified preparation of kievitone hydratase, the enzyme responsible for kievitone transformation, did not accomplish simultaneous detoxification; kievitone was detoxified, but phaseollidin was not.

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