Effect of thrombin on glycolysis of fresh and stored platelets.

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Abstract
Thrombin increases lactate concentration in citrate or ethylenediaminetetraacetate (EDTA) platelet-rich plasma and in glucose-containing suspensions of washed platelets. The increased rate of lactate formation does not require the addition of Ca ion, insulin, or fibrinogen to the washed platelet suspension. Lactate increase does not occur in the presence of heparin or when thrombin concentrations are too low to permit visible clotting of fibrinogen. Inability of fructose to substitute for glucose implies that platelets lack fructokinase. When glucose concentration is less than 2.5 [mu]moles/ 109 platelets at the time thrombin is added, subsequent clot retraction and lactate formation diminish correspondingly. Platelets stored in acid citrate dextrose (ACD) plasma and washed prior to testing undergo an exponential decrease in rate of glycolysis, with or without thrombin stimulation, during the first 72 hr of storage. Platelet adenosine triphosphate (ATP) concentration and ability to support clot retraction decrease at a slower rate.