Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria
Open Access
- 1 July 1996
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 23 (1) , 55-60
- https://doi.org/10.1111/j.1472-765x.1996.tb00028.x
Abstract
Repetitive element sequencebased PCR (rep-PCR) was used to generate DNA fingerprints for Listeria spp. Two primer sets (REP 1R-I REP 2-I and ERIC 1R ERIC 2) used in respectively REP-and ERIC-PCR revealed that bacteria of the genus Listeria possess short repetitive extragenic palindromic elements and enterobacterial repetitive intergenic consensus sequences. Specific band profiles obtained by ERIC-PCR enabled the identification of Listeria species. With both REP-and ERIC-PCR the L. monocytogenes serotypes 1/2a, 1/2b, 1/2c, 3b and 4b could be clearly distinguished from each other. Within the serotype 1/2a, REP-PCR showed a higher discriminative potential than ERIC-PCR and a comparable discriminative potential as RAPD combining 3-4 primers.Keywords
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