Quantification of proteins in sample buffer for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis using colloidal silver

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Abstract
A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1 μL of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5–100 ng.