DNA Vaccines for Influenza Virus: Differential Effects of Maternal Antibody on Immune Responses to Hemagglutinin and Nucleoprotein
- 1 September 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 74 (17) , 7787-7793
- https://doi.org/10.1128/jvi.74.17.7787-7793.2000
Abstract
Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active immunization, placing infants at risk for severe bacterial and viral infection. We tested the ability of intramuscular and gene gun immunization with DNA expressing influenza virus hemagglutinin (HA) and nucleoprotein (NP) to raise protective humoral and cellular responses in the presence or absence of maternal antibody. Neonatal mice born to influenza virus-immune mothers raised full antibody responses to NP but failed to generate antibody responses to HA. In contrast, the presence of maternal antibody did not affect the generation of long-lived CD8+ T-cell responses to both HA and NP. Thus, maternal antibody did not affect cell-mediated responses but did affect humoral responses, with the ability to limit the antibody response correlating with whether the DNA-expressed immunogen was localized in the plasma membrane or within the cell.Keywords
This publication has 31 references indexed in Scilit:
- Basic Mechanisms of DNA-Raised Antibody Responses to Intramuscular and Gene Gun ImmunizationsDNA and Cell Biology, 2000
- Immunization of Neonates with DNA Encoding a Bovine Herpesvirus Glycoprotein Is Effective in the Presence of Maternal Antibodies*Viral Immunology, 1999
- Influence of maternal antibodies on vaccine responses: inhibition of antibody but not T cell responses allows successful early prime-boost strategies in miceEuropean Journal of Immunology, 1998
- Enhanced protection against influenza virus of mice immunized as newborns with a mixture of plasmids expressing hemagglutinin and nucleoproteinVaccine, 1998
- Gene gun-based nucleic acid immunization: elicitation of humoral and cytotoxic T lymphocyte responses following epidermal delivery of nanogram quantities of DNAVaccine, 1995
- Heterologous and Homologous Protection Against Influenza A by DNA Vaccination: Optimization of DNA VectorsDNA and Cell Biology, 1993
- Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral ProteinScience, 1993
- Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cellsNature, 1990
- Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtypeNature, 1981
- Persistence of maternal antibody in infants beyond 12 months: Mechanism of measles vaccine failureThe Journal of Pediatrics, 1977