Prolactin induces growth inhibition and promotes differentiation of CHO cells stably transfected with prolactin receptor complementary DNA
- 16 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 358 (1) , 84-88
- https://doi.org/10.1016/0014-5793(94)01400-u
Abstract
We have characterized a stable and functional transfectant of the rabbit prolactin receptor in Chinese hamster ovary cells, and investigated the action of prolactin (PRL) on the growth and differentiation of this transfectant (clone E32). PRL induced a significant inhibition of E32 cell proliferation. Growth inhibition correlated with gene induction of the molecular marker of ovarian differentiation cholesterol side chain cleavage P450 (P450scc). Both effects were inversely proportional to cell confluence. The limits and potential development of such transfected cellular systems are discussed.Keywords
This publication has 15 references indexed in Scilit:
- Development of the mammary gland and lactationTrends in Endocrinology & Metabolism, 1994
- The Prolactin/Growth Hormone Receptor FamilyEndocrine Reviews, 1991
- Prolactin receptor gene expression in the rabbit: Identification, characterization and tissue distribution of several prolactin receptor messenger RNAs encoding a unique precursorMolecular and Cellular Endocrinology, 1991
- Growth Hormone (GH) Stimulates Protein Synthesis in Cells Transfected with GH Receptor Complementary DNAMolecular Endocrinology, 1990
- Growth hormone and adipose differentiation: growth hormone-induced antimitogenic state in 3T3-F442A preadipose cells.Proceedings of the National Academy of Sciences, 1990
- Induction and mitochondrial localization of cytochrome P450scc system enzymes in normal and transformed ovarian granulosa cells.The Journal of cell biology, 1990
- Prolactin stimulates milk protein promoter in CHO cells cotransfected with prolactin receptor cDNAMolecular and Cellular Endocrinology, 1990
- Immunological recognition of the prolactin receptor: identification of a single binding unit of molecular weight ≈42 000Biochimie, 1987
- Characterization of antisera to a partially purified prolactin receptor: effect on prolactin binding in different target tissuesMolecular and Cellular Endocrinology, 1984
- Evolution of Prolactin Receptors in Rabbit Mammary Gland During Pregnancy and LactationEndocrinology, 1977