Structure of a Cephalosporium acremonium mtDNA replicator
- 17 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 198 (1) , 92-98
- https://doi.org/10.1016/0014-5793(86)81191-7
Abstract
We have investigated the ARS (autonomously replicating sequence) activity of a 1.94 kb mitochondrial DNA fragment of Cephalosporium acremonium and found that several subfragments of this piece of mtDNA conferred the ARS phenotype. The nucleotide sequence of the fragment shows: (i) a high A + T content (72.5%); (ii) a perfect consensus ARS sequence (ATTTATATTTA) in the subfragment with the highest ARS activity; (iii) a large number of ARS consensus‐related sequences in the other subfragments, even in one lacking ARS activity; (iv) several potential hairpin structures. One of them contains the perfect consensus ARS sequence.Keywords
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