Thrombomucin, a Novel Cell Surface Protein that Defines Thrombocytes and Multipotent Hematopoietic Progenitors
Open Access
- 22 September 1997
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 138 (6) , 1395-1407
- https://doi.org/10.1083/jcb.138.6.1395
Abstract
MEP21 is an avian antigen specifically expressed on the surface of Myb-Ets–transformed multipotent hematopoietic precursors (MEPs) and of normal thrombocytes. Using nanoelectrospray tandem mass spectrometry, we have sequenced and subsequently cloned the MEP21 cDNA and named the gene thrombomucin as it encodes a 571–amino acid protein with an extracellular domain typical of the mucin family of proteoglycans. Thrombomucin is distantly related to CD34, the best characterized and most used human hematopoietic stem cell marker. It is also highly homologous in its transmembrane/intracellular domain to podocalyxinlike protein–1, a rabbit cell surface glycoprotein of kidney podocytes.Keywords
This publication has 67 references indexed in Scilit:
- HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors.The Journal of cell biology, 1996
- Analytical Properties of the Nanoelectrospray Ion SourceAnalytical Chemistry, 1996
- Self-renewal and differentiation of normal avian erythroid progenitor cells: Regulatory roles of the
TGFα c -ErbBSCF c -KitCell, 1993 - v-myb and v-ets transform chicken erythroid cells and cooperate both in trans and in cis to induce distinct differentiation phenotypes.Genes & Development, 1991
- A single point mutation in the v-ets oncogene affects both erythroid and myelomonocytic cell differentiationCell, 1988
- Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell.The Journal of cell biology, 1984
- Terminal differentiation of hemopoietic cell clones cultured in tridimensional collagen matrix: in situ cell morphology and enzyme histochemistry analysisBiology of the Cell, 1984
- Detection of avian hematopoietic cell surface antigens with monoclonal antibodies to myeloid cellsExperimental Cell Research, 1983
- Erythroblast cell lines transformed by a temperature-sensitive mutant of avian erythroblastosis virus: A model system to study erythroid differentiation in vitroJournal of Cellular Physiology, 1982
- Peroxidaseless Chicken Leukocytes: Isolation and Characterization of Antibacterial GranulesThe Journal of Infectious Diseases, 1973