Abstract
The complete utilization of choline and the accumulation of ammonia by resting cells of Achromobacter cholinophagum has been demonstrated. Betaine was isolated as betaine reineckate from incubation mixtures and shown to be the initial product of choline oxidation. Formaldehyde was demonstrated as an intermediate of betaine, dimethylglycine, or sarcosine oxidation. Serine was shown to be a metabolite and assayed quantitatively by oxidation with periodate. Pyruvic acid, formed from serine by cell-free extracts, was isolated as the 2,4-dinitrophenylhydrazone.